Pullulanase from rice endosperm.
نویسندگان
چکیده
منابع مشابه
Pullulanase from rice endosperm.
Pullulanase (EC 3.2.1.41) in non-germinating seeds was compared with that in germinating seeds. Moreover, pullulanase from the endosperm of rice (Oryza sativa L., cv. Hinohikari) seeds was isolated and its properties investigated. The pI value of pullulanase from seeds after 8 days of germination was almost equal to that from non-germinating seeds, which shows that these two enzymes are the sam...
متن کاملThe starch-debranching enzymes isoamylase and pullulanase are both involved in amylopectin biosynthesis in rice endosperm
The activities of the two types of starch debranching enzymes, isoamylase and pullulanase, were greatly reduced in endosperms of allelic sugary-1 mutants of rice (Oryza sativa), with the decrease more pronounced for isoamylase than for pullulanase. However, the decrease in isoamylase activity was not related to the magnitude of the sugary phenotype (the proportion of the phytoglycogen region of...
متن کاملStarch Biosynthesis in Rice Endosperm
© 2014 TERRAPUB, Tokyo. All rights reserved. doi:10.5047/agbm.2014.00401.0001 water (Fig. 1) or treating it with alkaline, urea, or dimethyl sulfoxide through the cutting of hydrogen bonds between the starch molecules. It is thought that digestion of hydrogen bonds results in the unwinding of the double helices of parallel α-1,4 glucan chains in the crystal lamellae of amylopectin molecules. Th...
متن کاملCharacterization of pullulanase (PUL)-deficient mutants of rice (Oryza sativa L.) and the function of PUL on starch biosynthesis in the developing rice endosperm
Rice (Oryza sativa) allelic sugary1 (sug1) mutants defective in isoamylase 1 (ISA1) accumulate varying levels of starch and phytoglycogen in their endosperm, and the activity of a pullulanase-type of a debranching enzyme (PUL) was found to correlate closely with the severity of the sug1 phenotype. Thus, three PUL-deficient mutants were generated to investigate the function of PUL in starch bios...
متن کاملMultiple Isoforms of Endosperm Sucrose Synthase from Guichao Rice
Sucrose synthase activity in rice is potentially yield limiting. This is because the carbon that forms starch enters the developing rice grain as sucrose and that sucrose must be broken down to form the precursors of starch. The enzyme that breaks down sucrose in the filling rice grain is sucrose synthase. Earlier research efforts on rice physiology indicated that endosperm sucrose-synthase act...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 2008
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.2008_3056